Review



avidin biotin horseradish peroxidase solution  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Vector Laboratories avidin biotin horseradish peroxidase solution
    Avidin Biotin Horseradish Peroxidase Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13030990-435-11-15
    Average 99 stars, based on 12897 article reviews
    avidin biotin horseradish peroxidase solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Projection Targeting with Phototagging to Study the Structure and Function of Retinal Ganglion Cells
    Article Snippet: The retinas were rinsed, then placed in a solution containing 5% normal donkey serum and biotinylated donkey anti-chicken IgY (1:500, Invitrogen; A16003) as the secondary antibody and incubated overnight. .. After rinsing with PBS, the retinas were incubated in a 1% Avidin-Biotin horseradish peroxidase solution (Vector Labs; PK-6100) for 1 hour. ..

    Article Title: Projection targeting with phototagging to study the structure and function of retinal ganglion cells.
    Article Snippet: The retinas were rinsed, then placed in a solution containing 5% normal donkey serum and biotinylated donkey anti-chicken IgY (1:500, Invitrogen; A16003) as the secondary antibody and incubated overnight. .. After rinsing with PBS, the retinas were incubated in a 1% Avidin-Biotin horseradish peroxidase solution (Vector Labs; PK6100) for 1 h. They were then rinsed again and transferred to a solution containing 1% cobalt chloride, 1% nickel ammonium sulfate, and diaminobenzidine tetrahydrochloride for 25 min. To catalyze the chromogen reaction, 3% hydrogen peroxide was added to this solution, and the retinas were incubated for an additional 45 min. ..

    Article Title: Effects of Testosterone and Its Major Metabolites upon Different Stages of Neuron Survival in the Dentate Gyrus of Male Rats
    Article Snippet: Tissue was next blocked for 30 min in a solution of 0.1 M TBS, 0.1% Triton-X 100, and 3.0% normal horse serum, followed by incubation for 16 h on a platform shaker at 4 °C in mouse monoclonal antibodies against BrdU (1:400 in blocking solution; Roche Diagnostics, Indianapolis, IN, USA). .. Sections were next incubated in horse anti-mouse secondary antibodies (1:100 in 0.1 M TBS; Vector Laboratories) for 4 h, followed by a 1.5 h incubation in avidin-biotin horseradish peroxidase solution (1:50; ABC Elite Kit, Vector Laboratories). .. Sections were reacted for 3–5 min in a solution of 3,3′diaminobenzidine (0.5 mg/mL; Sigma-Aldrich, Atlanta, GA, USA) and 0.003% H 2 O 2 in 0.1 M TBS and then mounted onto Superfrost Plus microscope slides (Fisher Scientific, Suwanee, GA, USA).

    Article Title: Effects of Testosterone and Its Major Metabolites upon Different Stages of Neuron Survival in the Dentate Gyrus of Male Rats.
    Article Snippet: Tissue was next blocked for 30 min in a solution of 0.1 M TBS, 0.1% Triton-X 100, and 3.0% normal horse serum, followed by incubation for 16 h on a platform shaker at 4 ◦C in mouse monoclonal antibodies against BrdU (1:400 in blocking solution; Roche Diagnostics, Indianapolis, IN, USA). .. Sections were next incubated in horse anti-mouse secondary antibodies (1:100 in 0.1 M TBS; Vector Laboratories) for 4 h, followed by a 1.5 h incubation in avidin-biotin horseradish peroxidase solution (1:50; ABC Elite Kit, Vector Laboratories). .. Sections were reacted for 3–5 min in a solution of 3,3′diaminobenzidine (0.5 mg/mL; Sigma-Aldrich, Atlanta, GA, USA) and 0.003% H2O2 in 0.1 M TBS and then mounted onto Superfrost Plus microscope slides (Fisher Scientific, Suwanee, GA, USA).

    Article Title: Desvenlafaxine May Accelerate Neuronal Maturation in the Dentate Gyri of Adult Male Rats
    Article Snippet: .. The following day, tissue was incubated in biotinylated SP anti-rat IgG (1∶500 in blocking solution; Jackson Immunoresearch; West Grove, PA) solution for 4 h at room before being complexed with avidin-biotin horseradish peroxidase solution (PK 6100; Vector Labs, Burlingame, CA) for 20 min and then developed in a solution of tris-buffered saline (TBS; pH 7.4) containing 0.02% diaminobenzene tetrahydrochloride (DAB) and 0.5% H 2 O 2 for 2–5 min. ..

    Article Title: Sex differences in inflammation in the hippocampus and amygdala across the lifespan in rats: associations with cognitive bias.
    Article Snippet: Sections were then washed another 3 × in 0.1 M PBS for 10 min per wash, and then incubated at 4 °C in DCX primary antibody (1:1000 goat Anti-DCX pAb; SC-8066; Santa Cruz Biotechnology, Dallas, TX, USA), 3% normal rabbit serum (VECTS5000, Vector Laboratories, Inc, Burlingame, CA), and 4% Triton-X in PBS for 24 h. The next day, sections were washed 5 × in 0.1 M PBS for 10 min per wash and incubated overnight at 4 °C in secondary antibody (biotinylated rabbit antigoat IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). .. The last day, after another series of 5 washes in 0.1 M PBS for 10 min per wash, sections were incubated in an avidin–biotin horseradish peroxidase solution (PK4000, Vector Laboratories, Inc, Burlingame, CA) for 4 h at room temperature. .. Sections were washed 3 × in 0.1 M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min.

    Article Title: Projection targeting with phototagging to study the structure and function of retinal ganglion cells
    Article Snippet: The retinas were rinsed, then placed in a solution containing 5% normal donkey serum and biotinylated donkey anti-chicken IgY (1:500, Invitrogen; A16003) as the secondary antibody and incubated overnight. .. After rinsing with PBS, the retinas were incubated in a 1% Avidin-Biotin horseradish peroxidase solution (Vector Labs; PK-6100) for 1 h. They were then rinsed again and transferred to a solution containing 1% cobalt chloride, 1% nickel ammonium sulfate, and diaminobenzidine tetrahydrochloride for 25 min. To catalyze the chromogen reaction, 3% hydrogen peroxide was added to this solution, and the retinas were incubated for an additional 45 min. ..

    Article Title: Neuroanatomical, electrophysiological, and morphological characterization of melanin-concentrating hormone cells coexpressing cocaine- and amphetamine-regulated transcript
    Article Snippet: .. The sections were then treated with an avidin-biotin-horseradish peroxidase solution (1:1:833; PK-6100, Vector Laboratories, Burlingame, CA; RRID: AB_2336819) for 30 min (RT), rinsed thrice with PBS (10 min each), and incubated (20 min) in a PBT solution comprising 0.005% hydrogen peroxide and 0.5% biotinylated (EZ-Link sulfo-NHS-LC-biotin; ThermoFisher Scientific) tyramine (Sigma-Aldrich, St. Louis, MO), which was made in-house ( ). ..

    Avidin-Biotin Assay:

    Article Title: Projection Targeting with Phototagging to Study the Structure and Function of Retinal Ganglion Cells
    Article Snippet: The retinas were rinsed, then placed in a solution containing 5% normal donkey serum and biotinylated donkey anti-chicken IgY (1:500, Invitrogen; A16003) as the secondary antibody and incubated overnight. .. After rinsing with PBS, the retinas were incubated in a 1% Avidin-Biotin horseradish peroxidase solution (Vector Labs; PK-6100) for 1 hour. ..

    Article Title: Projection targeting with phototagging to study the structure and function of retinal ganglion cells.
    Article Snippet: The retinas were rinsed, then placed in a solution containing 5% normal donkey serum and biotinylated donkey anti-chicken IgY (1:500, Invitrogen; A16003) as the secondary antibody and incubated overnight. .. After rinsing with PBS, the retinas were incubated in a 1% Avidin-Biotin horseradish peroxidase solution (Vector Labs; PK6100) for 1 h. They were then rinsed again and transferred to a solution containing 1% cobalt chloride, 1% nickel ammonium sulfate, and diaminobenzidine tetrahydrochloride for 25 min. To catalyze the chromogen reaction, 3% hydrogen peroxide was added to this solution, and the retinas were incubated for an additional 45 min. ..

    Article Title: Effects of Testosterone and Its Major Metabolites upon Different Stages of Neuron Survival in the Dentate Gyrus of Male Rats
    Article Snippet: Tissue was next blocked for 30 min in a solution of 0.1 M TBS, 0.1% Triton-X 100, and 3.0% normal horse serum, followed by incubation for 16 h on a platform shaker at 4 °C in mouse monoclonal antibodies against BrdU (1:400 in blocking solution; Roche Diagnostics, Indianapolis, IN, USA). .. Sections were next incubated in horse anti-mouse secondary antibodies (1:100 in 0.1 M TBS; Vector Laboratories) for 4 h, followed by a 1.5 h incubation in avidin-biotin horseradish peroxidase solution (1:50; ABC Elite Kit, Vector Laboratories). .. Sections were reacted for 3–5 min in a solution of 3,3′diaminobenzidine (0.5 mg/mL; Sigma-Aldrich, Atlanta, GA, USA) and 0.003% H 2 O 2 in 0.1 M TBS and then mounted onto Superfrost Plus microscope slides (Fisher Scientific, Suwanee, GA, USA).

    Article Title: Effects of Testosterone and Its Major Metabolites upon Different Stages of Neuron Survival in the Dentate Gyrus of Male Rats.
    Article Snippet: Tissue was next blocked for 30 min in a solution of 0.1 M TBS, 0.1% Triton-X 100, and 3.0% normal horse serum, followed by incubation for 16 h on a platform shaker at 4 ◦C in mouse monoclonal antibodies against BrdU (1:400 in blocking solution; Roche Diagnostics, Indianapolis, IN, USA). .. Sections were next incubated in horse anti-mouse secondary antibodies (1:100 in 0.1 M TBS; Vector Laboratories) for 4 h, followed by a 1.5 h incubation in avidin-biotin horseradish peroxidase solution (1:50; ABC Elite Kit, Vector Laboratories). .. Sections were reacted for 3–5 min in a solution of 3,3′diaminobenzidine (0.5 mg/mL; Sigma-Aldrich, Atlanta, GA, USA) and 0.003% H2O2 in 0.1 M TBS and then mounted onto Superfrost Plus microscope slides (Fisher Scientific, Suwanee, GA, USA).

    Article Title: Desvenlafaxine May Accelerate Neuronal Maturation in the Dentate Gyri of Adult Male Rats
    Article Snippet: .. The following day, tissue was incubated in biotinylated SP anti-rat IgG (1∶500 in blocking solution; Jackson Immunoresearch; West Grove, PA) solution for 4 h at room before being complexed with avidin-biotin horseradish peroxidase solution (PK 6100; Vector Labs, Burlingame, CA) for 20 min and then developed in a solution of tris-buffered saline (TBS; pH 7.4) containing 0.02% diaminobenzene tetrahydrochloride (DAB) and 0.5% H 2 O 2 for 2–5 min. ..

    Article Title: Sex differences in inflammation in the hippocampus and amygdala across the lifespan in rats: associations with cognitive bias.
    Article Snippet: Sections were then washed another 3 × in 0.1 M PBS for 10 min per wash, and then incubated at 4 °C in DCX primary antibody (1:1000 goat Anti-DCX pAb; SC-8066; Santa Cruz Biotechnology, Dallas, TX, USA), 3% normal rabbit serum (VECTS5000, Vector Laboratories, Inc, Burlingame, CA), and 4% Triton-X in PBS for 24 h. The next day, sections were washed 5 × in 0.1 M PBS for 10 min per wash and incubated overnight at 4 °C in secondary antibody (biotinylated rabbit antigoat IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). .. The last day, after another series of 5 washes in 0.1 M PBS for 10 min per wash, sections were incubated in an avidin–biotin horseradish peroxidase solution (PK4000, Vector Laboratories, Inc, Burlingame, CA) for 4 h at room temperature. .. Sections were washed 3 × in 0.1 M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min.

    Article Title: Projection targeting with phototagging to study the structure and function of retinal ganglion cells
    Article Snippet: The retinas were rinsed, then placed in a solution containing 5% normal donkey serum and biotinylated donkey anti-chicken IgY (1:500, Invitrogen; A16003) as the secondary antibody and incubated overnight. .. After rinsing with PBS, the retinas were incubated in a 1% Avidin-Biotin horseradish peroxidase solution (Vector Labs; PK-6100) for 1 h. They were then rinsed again and transferred to a solution containing 1% cobalt chloride, 1% nickel ammonium sulfate, and diaminobenzidine tetrahydrochloride for 25 min. To catalyze the chromogen reaction, 3% hydrogen peroxide was added to this solution, and the retinas were incubated for an additional 45 min. ..

    Article Title: Neuroanatomical, electrophysiological, and morphological characterization of melanin-concentrating hormone cells coexpressing cocaine- and amphetamine-regulated transcript
    Article Snippet: .. The sections were then treated with an avidin-biotin-horseradish peroxidase solution (1:1:833; PK-6100, Vector Laboratories, Burlingame, CA; RRID: AB_2336819) for 30 min (RT), rinsed thrice with PBS (10 min each), and incubated (20 min) in a PBT solution comprising 0.005% hydrogen peroxide and 0.5% biotinylated (EZ-Link sulfo-NHS-LC-biotin; ThermoFisher Scientific) tyramine (Sigma-Aldrich, St. Louis, MO), which was made in-house ( ). ..

    Blocking Assay:

    Article Title: Desvenlafaxine May Accelerate Neuronal Maturation in the Dentate Gyri of Adult Male Rats
    Article Snippet: .. The following day, tissue was incubated in biotinylated SP anti-rat IgG (1∶500 in blocking solution; Jackson Immunoresearch; West Grove, PA) solution for 4 h at room before being complexed with avidin-biotin horseradish peroxidase solution (PK 6100; Vector Labs, Burlingame, CA) for 20 min and then developed in a solution of tris-buffered saline (TBS; pH 7.4) containing 0.02% diaminobenzene tetrahydrochloride (DAB) and 0.5% H 2 O 2 for 2–5 min. ..

    Saline:

    Article Title: Desvenlafaxine May Accelerate Neuronal Maturation in the Dentate Gyri of Adult Male Rats
    Article Snippet: .. The following day, tissue was incubated in biotinylated SP anti-rat IgG (1∶500 in blocking solution; Jackson Immunoresearch; West Grove, PA) solution for 4 h at room before being complexed with avidin-biotin horseradish peroxidase solution (PK 6100; Vector Labs, Burlingame, CA) for 20 min and then developed in a solution of tris-buffered saline (TBS; pH 7.4) containing 0.02% diaminobenzene tetrahydrochloride (DAB) and 0.5% H 2 O 2 for 2–5 min. ..



    Similar Products

    99
    Vector Laboratories avidin biotin horseradish peroxidase solution
    Avidin Biotin Horseradish Peroxidase Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13030990-435-11-15
    Average 99 stars, based on 1 article reviews
    avidin biotin horseradish peroxidase solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    93
    Vector Laboratories avidin biotin horseradish peroxidase complex solution
    Avidin Biotin Horseradish Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+solution/Avidin+D%2C+Horseradish+Peroxidase+(Av-HRP)%2C+Concentrate%2C+for+IHC/bio_rxiv__2025__08__05__668723-73-14-23
    Average 93 stars, based on 1 article reviews
    avidin biotin horseradish peroxidase complex solution - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    99
    Vector Laboratories horseradish peroxidase hrp conjugated avidin biotin complex solution
    Horseradish Peroxidase Hrp Conjugated Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm39303715-743-33-44
    Average 99 stars, based on 1 article reviews
    horseradish peroxidase hrp conjugated avidin biotin complex solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    94
    Vector Laboratories avidin–biotin horseradish peroxidase abc complex solution
    Avidin–Biotin Horseradish Peroxidase Abc Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+solution/DAB+Enhancing+Solution/10__1523_slash_eneuro__0424___23__2023-70-26-33
    Average 94 stars, based on 1 article reviews
    avidin–biotin horseradish peroxidase abc complex solution - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Vector Laboratories avidin-biotin-horseradish peroxidase solution
    Avidin Biotin Horseradish Peroxidase Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+solution/DAB+Enhancing+Solution/pmc10695847-64-6-9
    Average 94 stars, based on 1 article reviews
    avidin-biotin-horseradish peroxidase solution - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results